Exogenous ethylene stimulates the long-term expression of genes related to anthocyanin biosynthesis in grape berries
Identifieur interne : 00B196 ( Main/Exploration ); précédent : 00B195; suivant : 00B197Exogenous ethylene stimulates the long-term expression of genes related to anthocyanin biosynthesis in grape berries
Auteurs : Ashraf El-Kereamy [France] ; Christian Chervin [France] ; Jean-Paul Roustan [France] ; Veronique Cheynier [France] ; Jean-Marc Souquet [France] ; Michel Moutounet [France] ; José Raynal [France] ; Christopher Ford [Australie] ; Alain Latche [France] ; Jean-Claude Pech [France] ; Mondher Bouzayen [France]Source :
- Physiologia Plantarum : (København. 1948) [ 0031-9317 ] ; 2003.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Fruit.
English descriptors
- KwdEn :
Abstract
The treatment of grape berries (Vitis vinifera L. cv. Cabernet Sauvignon) with the ethylene-releasing compound, 2-chloro-ethylphosphonic acid (2-CEPA), at veraison is a method known to enhance grape skin colour. We observed that it produced a 6-fold increase, up to 30 pmol g-1 FW, of the cluster internal ethylene compared to untreated controls within the 24 h following treatment. This ethylene upsurge was associated with increased levels of chalcone synthase (CHS) and flavanone 3-hydroxylase (F3H) transcripts, which persisted over the following 20 days. Transcript levels of leucoanthocyanidin dioxygenase (LDOX) and UDP glucose-flavonoid 3-O-glucosyl transferase (UFGT) were similarly enhanced by 2-CEPA, although to a lesser extent. The effect on UFGT was confirmed at the protein level by an immunoblot analysis. The transcript accumulation of dihydro-flavonol 4-reductase (DFR) was unaffected by 2-CEPA treatment. Examination of the levels of CHS, F3H and UFGT mRNAs in berries during bunch exposure to ethylene, revealed elevated levels of each transcript within the first 6 h of treatment when compared to nonethylene-treated controls. HPLC analyses of berry skin extracts showed that levels of each of the anthocyanins analysed (delphinidin, cyanidin, petunidin, peonidin and malvidin) increased over the 10 days following the ethylene burst, and decreased thereafter. However, anthocyanin levels at harvest were still higher in ethylene treated grapes than in controls. This data is the first evidence that ethylene triggers gene expression related to anthocyanin synthesis in grapes, and in addition, our results also confirm the existence of other regulatory modes in the anthocyanin biosynthetic pathway.
Affiliations:
- Australie, France
- Languedoc-Roussillon, Midi-Pyrénées, Occitanie (région administrative)
- Castanet, Montpellier
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berries</title><author><name sortKey="El Kereamy, Ashraf" sort="El Kereamy, Ashraf" uniqKey="El Kereamy A" first="Ashraf" last="El-Kereamy">Ashraf El-Kereamy</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>UMR 990, Genomique et Biotechnologie des Fruits, INRA-INP/ENSA Toulouse, BP 107</s1><s2>31326 Castanet</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet</settlement></placeName></affiliation></author><author><name sortKey="Chervin, Christian" sort="Chervin, Christian" uniqKey="Chervin C" first="Christian" last="Chervin">Christian Chervin</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>UMR 990, Genomique et Biotechnologie des Fruits, INRA-INP/ENSA Toulouse, BP 107</s1><s2>31326 Castanet</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet</settlement></placeName></affiliation></author><author><name sortKey="Roustan, Jean Paul" sort="Roustan, Jean Paul" uniqKey="Roustan J" first="Jean-Paul" last="Roustan">Jean-Paul Roustan</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>UMR 990, Genomique et Biotechnologie des Fruits, INRA-INP/ENSA Toulouse, BP 107</s1><s2>31326 Castanet</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet</settlement></placeName></affiliation></author><author><name sortKey="Cheynier, Veronique" sort="Cheynier, Veronique" uniqKey="Cheynier V" first="Veronique" last="Cheynier">Veronique Cheynier</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>UMR Sciences pour l'Œnologie, Laboratoire Biopolymères et Aromes, INRA, place Viala</s1><s2>34060 Montpellier</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Souquet, Jean Marc" sort="Souquet, Jean Marc" uniqKey="Souquet J" first="Jean-Marc" last="Souquet">Jean-Marc Souquet</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>UMR Sciences pour l'Œnologie, Laboratoire Biopolymères et Aromes, INRA, place Viala</s1><s2>34060 Montpellier</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Moutounet, Michel" sort="Moutounet, Michel" uniqKey="Moutounet M" first="Michel" last="Moutounet">Michel Moutounet</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>UMR Sciences pour l'Œnologie, Laboratoire Biopolymères et Aromes, INRA, place Viala</s1><s2>34060 Montpellier</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Raynal, Jose" sort="Raynal, Jose" uniqKey="Raynal J" first="José" last="Raynal">José Raynal</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>UMR 990, Genomique et Biotechnologie des Fruits, INRA-INP/ENSA Toulouse, BP 107</s1><s2>31326 Castanet</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet</settlement></placeName></affiliation></author><author><name sortKey="Ford, Christopher" sort="Ford, Christopher" uniqKey="Ford C" first="Christopher" last="Ford">Christopher Ford</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>School of Agriculture and Wine, The University of Adelaide, PMB 1</s1><s2>Glen Osmond SA 5064</s2><s3>AUS</s3><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Glen Osmond SA 5064</wicri:noRegion></affiliation></author><author><name sortKey="Latche, Alain" sort="Latche, Alain" uniqKey="Latche A" first="Alain" last="Latche">Alain Latche</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>UMR 990, Genomique et Biotechnologie des Fruits, INRA-INP/ENSA Toulouse, BP 107</s1><s2>31326 Castanet</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>31326 Castanet</wicri:noRegion><wicri:noRegion>BP 107</wicri:noRegion><wicri:noRegion>31326 Castanet</wicri:noRegion></affiliation></author><author><name sortKey="Pech, Jean Claude" sort="Pech, Jean Claude" uniqKey="Pech J" first="Jean-Claude" last="Pech">Jean-Claude Pech</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>UMR 990, Genomique et Biotechnologie des Fruits, INRA-INP/ENSA Toulouse, BP 107</s1><s2>31326 Castanet</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>31326 Castanet</wicri:noRegion><wicri:noRegion>BP 107</wicri:noRegion><wicri:noRegion>31326 Castanet</wicri:noRegion></affiliation></author><author><name sortKey="Bouzayen, Mondher" sort="Bouzayen, Mondher" uniqKey="Bouzayen M" first="Mondher" last="Bouzayen">Mondher Bouzayen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>UMR 990, Genomique et Biotechnologie des Fruits, INRA-INP/ENSA Toulouse, BP 107</s1><s2>31326 Castanet</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>7 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>31326 Castanet</wicri:noRegion><wicri:noRegion>BP 107</wicri:noRegion><wicri:noRegion>31326 Castanet</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Physiologia Plantarum : (København. 1948)</title><title level="j" type="abbreviated">Physiol. Plant. : (Kbh., 1948)</title><idno type="ISSN">0031-9317</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Physiologia Plantarum : (København. 1948)</title><title level="j" type="abbreviated">Physiol. Plant. : (Kbh., 1948)</title><idno type="ISSN">0031-9317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anthocyanin</term><term>Biosynthesis</term><term>Ethylene</term><term>Fruit</term><term>Gene expression</term><term>Growth regulator treatment</term><term>Naringenin 3-dioxygenase</term><term>Naringenin-chalcone synthase</term><term>Vitis vinifera</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Traitement substance croissance</term><term>Anthocyane</term><term>Biosynthèse</term><term>Fruit</term><term>Naringenin 3-dioxygenase</term><term>Naringenin-chalcone synthase</term><term>Expression génique</term><term>Vitis vinifera</term><term>Ethylène</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Fruit</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The treatment of grape berries (Vitis vinifera L. cv. Cabernet Sauvignon) with the ethylene-releasing compound, 2-chloro-ethylphosphonic acid (2-CEPA), at veraison is a method known to enhance grape skin colour. We observed that it produced a 6-fold increase, up to 30 pmol g-1 FW, of the cluster internal ethylene compared to untreated controls within the 24 h following treatment. This ethylene upsurge was associated with increased levels of chalcone synthase (CHS) and flavanone 3-hydroxylase (F3H) transcripts, which persisted over the following 20 days. Transcript levels of leucoanthocyanidin dioxygenase (LDOX) and UDP glucose-flavonoid 3-O-glucosyl transferase (UFGT) were similarly enhanced by 2-CEPA, although to a lesser extent. The effect on UFGT was confirmed at the protein level by an immunoblot analysis. The transcript accumulation of dihydro-flavonol 4-reductase (DFR) was unaffected by 2-CEPA treatment. Examination of the levels of CHS, F3H and UFGT mRNAs in berries during bunch exposure to ethylene, revealed elevated levels of each transcript within the first 6 h of treatment when compared to nonethylene-treated controls. HPLC analyses of berry skin extracts showed that levels of each of the anthocyanins analysed (delphinidin, cyanidin, petunidin, peonidin and malvidin) increased over the 10 days following the ethylene burst, and decreased thereafter. However, anthocyanin levels at harvest were still higher in ethylene treated grapes than in controls. This data is the first evidence that ethylene triggers gene expression related to anthocyanin synthesis in grapes, and in addition, our results also confirm the existence of other regulatory modes in the anthocyanin biosynthetic pathway.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li></region><settlement><li>Castanet</li><li>Montpellier</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="El Kereamy, Ashraf" sort="El Kereamy, Ashraf" uniqKey="El Kereamy A" first="Ashraf" last="El-Kereamy">Ashraf El-Kereamy</name></region><name sortKey="Bouzayen, Mondher" sort="Bouzayen, Mondher" uniqKey="Bouzayen M" first="Mondher" last="Bouzayen">Mondher Bouzayen</name><name sortKey="Chervin, Christian" sort="Chervin, Christian" uniqKey="Chervin C" first="Christian" last="Chervin">Christian Chervin</name><name sortKey="Cheynier, Veronique" sort="Cheynier, Veronique" uniqKey="Cheynier V" first="Veronique" last="Cheynier">Veronique Cheynier</name><name sortKey="Latche, Alain" sort="Latche, Alain" uniqKey="Latche A" first="Alain" last="Latche">Alain Latche</name><name sortKey="Moutounet, Michel" sort="Moutounet, Michel" uniqKey="Moutounet M" first="Michel" last="Moutounet">Michel Moutounet</name><name sortKey="Pech, Jean Claude" sort="Pech, Jean Claude" uniqKey="Pech J" first="Jean-Claude" last="Pech">Jean-Claude Pech</name><name sortKey="Raynal, Jose" sort="Raynal, Jose" uniqKey="Raynal J" first="José" last="Raynal">José Raynal</name><name sortKey="Roustan, Jean Paul" sort="Roustan, Jean Paul" uniqKey="Roustan J" first="Jean-Paul" last="Roustan">Jean-Paul Roustan</name><name sortKey="Souquet, Jean Marc" sort="Souquet, Jean Marc" uniqKey="Souquet J" first="Jean-Marc" last="Souquet">Jean-Marc Souquet</name></country><country name="Australie"><noRegion><name sortKey="Ford, Christopher" sort="Ford, Christopher" uniqKey="Ford C" first="Christopher" last="Ford">Christopher Ford</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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